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Optimization of the host/vector system and culture conditions for production of penicillin acylase in Escherichia coli

✍ Scribed by C.Perry Chou; Bao-Yuan Kuo; Wen-Jer Lin

Book ID
114399922
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
843 KB
Volume
88
Category
Article
ISSN
1389-1723

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation