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A biochemical engineering approach for enhancing production of recombinant penicillin acylase inEscherichia coli

✍ Scribed by W.-J. Lin; B.-Y. Kuo; C. Chou

Publisher
Springer
Year
2001
Tongue
English
Weight
211 KB
Volume
24
Category
Article
ISSN
1615-7605

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation