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An adaptive response to alkylating agents inAspergillus nidulans

โœ Scribed by P. Hooley; S. G. Shawcross; P. Strike

Book ID
104790518
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
444 KB
Volume
14
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) could also adapt mycelium to later MNNG challenge, while 4NQO and UV could not. The inducible effect was not transmissible through conidia. A standard reversion assay based upon methG proved impractical for studying mutation frequencies during alkylation treatments owing to variations in MNNG resistance amongst revertants.

๐Ÿ“œ SIMILAR VOLUMES

Two classes of Bacillus subtilis mutants
Two classes of Bacillus subtilis mutants deficient in the adaptive response to simple alkylating agents
โœ Morohoshi, Fumiko ;Munakata, Nobuo ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 705 KB

Six mutant strains of Bacillus subtilis hypersensitive to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were shown to be deficient in the adaptive response to MNNG and termed ada mutants (Morohoshi and Munakata 1985). All the mutations mapped between the attSPO2 and lin loci on the chromosome. The mut