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Amplification of c-myc oncogene and absence of c-Ha-ras point mutation in human bone sarcoma

✍ Scribed by Dr. Carlos Barrios; Javier S. Castresana; Juan Ruiz; Andris Kreicbergs

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 733 KB
Volume: 11
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100110410

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The genomic organization of four oncogenes, c‐myc, c‐myb, c‐Ha‐ras, and v‐fms, was analyzed in 21 patients with malignant bone tumors. Amplification of the c‐myc proto‐oncogene without rearrangement was the sole abnormality detected in four tumors: two chondrosarcomas, one osteosarcoma, and one lymphoma of bone. DNA hybridizations with c‐myb, c‐Ha‐ras, and v‐fms probes disclosed no structural gene abnormalities. Point mutations at the 12th codon of the c‐Ha‐ras gene were investigated with the polymerase chain reaction technique; no alterations were detected. The observed amplification of the c‐myc there was not related to histologic type, grade, surgical stage, or ploidy level of the tumors. The results indicated that c‐myc amplification, presumed to be involved in the development of malignancy in a variety of solid tumors, is encountered sporadically in malignant bone tumors; however, this occurs without relation to common histopathologic features. The clinical significance of oncogene amplification in bone sarcoma remains to be established.

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