A simple and rapid procedure for the purification of plasmids from Escherichia coli K12 has been developed. Bacterial cells are subjected to the boiling procedure [D. S. Holmes, and M. Quigfey Anal. Biochem. 114,[193][194][195][196][197]] followed by removal of contaminating RNA by chromatography on
Amplification of bacterial plasmids without blocking protein biosynthesis
β Scribed by Ivo Angelov; Ivan Ivanov
- Book ID
- 119078991
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 265 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0147-619X
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