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Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector

✍ Scribed by Teather, Ron M. ;Müller-Hill, Benno ;Abrutsch, Ursula ;Aichele, Gabriele ;Overath, Peter

Publisher
Springer
Year
1978
Tongue
English
Weight
932 KB
Volume
159
Category
Article
ISSN
0026-8925

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✦ Synopsis

The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions where lactose carrier protein comprises 1.4% of the cytoplasmic membrane protein.

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