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Induction of prophage λ without amplification of recA protein

✍ Scribed by Baluch, John ;Sussman, Raquel ;Resnick, James

Publisher
Springer
Year
1980
Tongue
English
Weight
547 KB
Volume
178
Category
Article
ISSN
0026-8925

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✦ Synopsis

The requirement for amplified synthesis of recA protein in the UV-promoted induction of coliphage lambda was studied. We confirmed that a low concentration of rifampicin inhibited specifically the increased synthesis of recA protein after an inducing treatment (Satta and Pardee, 1978). Under these conditions, using an optimal dose of UV, E. coli lysogens were induced, producing active phage. The drug delayed the onset of induction and with increasing concentrations affected the yield of phage, but all the cells lysed. The results established that induction can proceed without amplification of recA protein synthesis.

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