✦ LIBER ✦
Rapid purification of bacterial plasmids and coliphage M13 RF without CsCl centrifugation
✍ Scribed by D.S. Holmes
- Book ID
- 102984607
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 785 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple and rapid procedure for the purification of plasmids from Escherichia coli K12 has been developed. Bacterial cells are subjected to the boiling procedure [D. S. Holmes, and M. Quigfey Anal. Biochem. 114,[193][194][195][196][197]] followed by removal of contaminating RNA by chromatography on Sepharose 2B and ofgenomic DNA by acid-phenol extraction. Plasmids are recovered with good yield. They can be restricted and ligated and will transform host cells. A simple modification of the procedure allows it to be used for the isolation of coliphage M 13 RF DNA.