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Affinity chromatography of collagen glucosyltransferase on a UDP-glucose derivative coupled to agarose

โœ Scribed by Henrik Anttinen; Kari I. Kivirikko


Book ID
115734369
Publisher
Elsevier Science
Year
1976
Weight
541 KB
Volume
429
Category
Article
ISSN
0005-2744

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Purification of a UTP: d-Glucose-1-phosp
โœ G. Azzar; F. Persat; A. Degiuli; R. Got ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 379 KB

UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characte