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Purification of 2-deoxy-2-dansylamido-D-glucose by affinity chromatography on a lectin-loaded agarose column

โœ Scribed by W. Bessler; P. Schindler


Book ID
112660787
Publisher
Springer
Year
1979
Tongue
English
Weight
228 KB
Volume
35
Category
Article
ISSN
1420-682X

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Purification of a UTP: d-Glucose-1-phosp
โœ G. Azzar; F. Persat; A. Degiuli; R. Got ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 379 KB

UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characte