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Coupling of a New, Active Morphine Derivative to Sepharose for Affinity Chromatography

✍ Scribed by Eric J. Simon, William P. Dole and Jacob M. Hiller

Book ID
123651190
Publisher
National Academy of Sciences
Year
1972
Tongue
English
Weight
552 KB
Volume
69
Category
Article
ISSN
0027-8424

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A new purification procedure for spinach leaf fructose-l,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/rag and a yield of 23%. The met