✦ LIBER ✦
A new procedure for the purification of spinach leaf photosynthetic fructose-1,6-bisphosphatase by affinity chromatography on mercaptoethylamine-Sepharose
✍ Scribed by A. Plá; A Chueca; J López-Gorgé
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 495 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0166-8595
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✦ Synopsis
A new purification procedure for spinach leaf fructose-l,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/rag and a yield of 23%. The method may also be useful for the purification of other thioredoxin-activated chloroplast enzymes.