✦ LIBER ✦

Affinity chromatography of carbonic anhydrase

✍ Scribed by Philip L. Whitney

Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 511 KB
Volume: 57
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90102-x

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

An improved method for the purification

An improved method for the purification of carbonic anhydrase isozymes by affinity chromatography

✍ William R.A. Osborne; Richard E. Tashian 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 590 KB

Therapeutic applications of glycosidic c

Therapeutic applications of glycosidic carbonic anhydrase inhibitors

✍ Jean-Yves Winum; Sally-Ann Poulsen;; Claudiu T. Supuran 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 390 KB

## Abstract The zinc enzymes carbonic anhydrases (CAs, EC 4.2.1.1) are very efficient catalysts for the reversible hydration of carbon dioxide to bicarbonate and hence play an important physiological role. In humans, 16 different isozymes have been described, some of them being involved in various

Dissociation constants for carbonic anhy

Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography

✍ William R.A. Osborne; Richard E. Tashian 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 401 KB

Carbonic anhydrase-azosulfonamide dissociation constants (Kd) were determined by gel filtration with high-performance liquid chromatography. By measuring the area of the elution peak at two wavelengths, Kd values were derived without having to measure a shallow trough. The procedure proved to be fas

Fluorescence analysis of sulfonamide bin

Fluorescence analysis of sulfonamide binding to carbonic anhydrase

✍ Sheila C. Wang; Deborah B. Zamble 📂 Article 📅 2006 🏛 The American Society for Biochemistry and Molecula 🌐 English ⚖ 349 KB 👁 2 views

## Abstract A practical laboratory experiment is described that illustrates the application of fluorescence resonance energy transfer to the study of protein‐ligand binding. The affinities of wild‐type and mutant human carbonic anhydrase II for dansylamide were determined by monitoring the increase

Simple assay for determination of carbon

Simple assay for determination of carbonic anhydrase activity

✍ Jerry L. Leeling; Barrie M. Phillips 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 English ⚖ 171 KB

N4-(5-Nitro-2-pyridyl)-4-homosulfanilamide-A mixture of 2chloro-5-nitropyridine (0.25 g.), 4-homosulfanilamide hydrochloride (0.32 g.), and freshly fused sodium acetate (0.2 8.) was refluxed with 30 ml. absolute ethanol for 24 hr., after which the reaction mixture was diluted with water until comple

Tandem mass specatrometry of carbonic an

Tandem mass specatrometry of carbonic anhydrase (29 kDa)

✍ Peter B. O'Connor; J. Paul Speir; Michael W. Senko; Daniel P. Little; Fred W. Mc 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 520 KB

Using electrospray ionization with a Fourier-transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings. Such sensitive and specific direct characterization of proteins ap