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Tandem mass specatrometry of carbonic anhydrase (29 kDa)

✍ Scribed by Peter B. O'Connor; J. Paul Speir; Michael W. Senko; Daniel P. Little; Fred W. McLafferty

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
520 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Using electrospray ionization with a Fourier-transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings. Such sensitive and specific direct characterization of proteins appears to be complementary to conventional techniques including LC/MS/MS sequencing of oligopeptides from protein degradation.

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