Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography

✍ Scribed by William R.A. Osborne; Richard E. Tashian

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 401 KB
• Volume: 137
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90089-7

✦ Synopsis

Carbonic anhydrase-azosulfonamide dissociation constants (Kd) were determined by gel filtration with high-performance liquid chromatography. By measuring the area of the elution peak at two wavelengths, Kd values were derived without having to measure a shallow trough. The procedure proved to be fast and reliable and has a general application. The dissociation constants were measured for 7-acetamido-2-(4'-sulfamylphenylazo)-1-hydroxynaphthalene-3, 6-disulfonate (Neoprontosil) complexes with carbonic anhydrase isozymes CA I, CA II, and CA III from bovine and human sources, and chicken CA III from skeletal muscle.