Simple assay for determination of carbonic anhydrase activity

✍ Scribed by Jerry L. Leeling; Barrie M. Phillips

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 171 KB
• Volume: 58
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600580735

✦ Synopsis

N4-(5-Nitro-2-pyridyl)-4-homosulfanilamide-A mixture of 2chloro-5-nitropyridine (0.25 g.), 4-homosulfanilamide hydrochloride (0.32 g.), and freshly fused sodium acetate (0.2 8.) was refluxed with 30 ml. absolute ethanol for 24 hr., after which the reaction mixture was diluted with water until complete separation. The product which was obtained in 60% yield (0.3 g.) melted at 190" after being crystallized from ethanol.

Anal.-Calcd. for CI2Hl2N4O4S: N, 18.30. Found: N, 17.97.

N-(5-Nitro-2-pyridyl)-benzylsulfonamide-A mixture of 2-chloro-5-nitropyridine (0.8 g.), potassium benzyl sulfonamide (0.9 g.) and dimethyl formamide (30 ml.) was heated at 110-130" for 3 hr. The reaction mixture was then evaporated under diminished pressure nearly to dryness, and the residue (1 g., 62% yield) was crystallized from ethanol. It melted at 260" and the melting point was not depressed on admixture with a pure specimen prepared by Ullmann's method.