An Assay for Carbonic Anhydrase Activity and Reactions That Produce Radiolabeled Gases or Small Uncharged Molecules
✍ Scribed by A. Stemler
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 364 KB
- Volume
- 210
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
An assay procedure for carbonic anhydrase activity is described. Unique to this method is the use of radiolabeled bicarbonate and a reaction vessel with a gas-permeable membrane. The method detects ({ }^{14} \mathrm{CO}{2}) production directly and can be used at room temperature and with solutions buffered over a wide pH range. Assays are rapid ( (5-10 \mathrm{~s}) ), discounting elution and scintillation counting time. The equipment required is simple and inexpensive, making the method ideal for routine determinations of carbonic anhydrase activity. The method can also be applied to other reactions that liberate radioactive (\mathrm{CO}{2}), other labeled gases, or small uncharged, labeled molecules. 1993 Academic Press, Inc.