Aeration-agitation studies on the novobiocin fermentation

✍ Scribed by Maxon, W. D.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1959
• Weight: 611 KB
• Volume: 1
• Category: Article
• ISSN: 0368-1467
• DOI: 10.1002/jbmte.390010308

✦ Synopsis

The. llpjolm C'ompniiy, Kalnmuzoo, .IIicliiyrcn Suninmy. Novobiocin fermentations in 20 1. baffled fermentors were studied as a function of the size and speed of the dual four-bladed flat-blade t.urbines used. Power input and sulphite oxidation rate measurements were made. The courses of pH, sugar utilization, mycelial dry weight,, a r b o n dioxide evolution and antibiotic titer were determined. Optimum antibiotic yields were achieved at a power input of 0.5 h.p./100 gal, equivalent t o a sulphite oxidation rate of 110 mmoles Oz/l.h, when the impeller diameters were 29 per cent or 39 per cent of the tank diameter. A power input of 0.75 h.p./100 gal, equivalent to a sulphite oxidation rate of 160 mmoles Oz/l.h, was required for equivalent results with the impeller diameter 49 per cent of the tank diameter. Some explanations of the lack of equivalent results with the large impeller are discussed.

* Smith33 has recently completed some shaken flask studies on aeration effects in the novobiocin fermentation. rrhntration and wration x i t h rcqwct to it5 influcnw on novobiocm titers He studied the interaction of mycelium con