Direct selection for recombinants by supplemented minimal media from polyethylene-glycol (PEG)-induced fusion of protoplasts of polyauxotrophic strains of B. megaterium revealed striking physiological influences on the yield of recombinants. Cytoplasmic state of the protoplasts to be fused, rather t
✦ LIBER ✦
Adventures with Bacillus megaterium – fusion of bacterial protoplasts
✍ Scribed by Lajos Alföldi
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 746 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0265-9247
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Polyethylene-glycol induced fusion of ba
✍
Fodor, Katalin ;Alf�ldi, Lajos
📂
Article
📅
1979
🏛
Springer
🌐
English
⚖ 451 KB
Genetic mapping by means of protoplast f
✍
Akamatsu, T. ;Sekiguchi, J.
📂
Article
📅
1987
🏛
Springer
🌐
English
⚖ 903 KB
A new mapping method involving protoplast fusion in Bacillus subtilis is described. Protoplasts from an isogenic standard marker strain containing purA and from a strain containing both purB and the marker, "x", to be mapped were fused with polyethylene glycol, and purA+ purB+ fusants were selected.
Polyethyleneglycol-induced transformatio
✍
Lévi-Meyrueis, Corinne ;Fodor, Katalin ;Schaeffer, Pierre
📂
Article
📅
1980
🏛
Springer
🌐
English
⚖ 538 KB
Direct selection of complementing diploi
✍
Sanchez-Rivas, Carmen
📂
Article
📅
1982
🏛
Springer
🌐
English
⚖ 494 KB
Diploid state of phenotypically recombin
✍
Sanchez-Rivas, Carmen ;Lévi-Meyrueis, Corinne ;Lazard-Monier, Florence ;Schaeffe
📂
Article
📅
1982
🏛
Springer
🌐
English
⚖ 785 KB
Transfection of protoplasts of Bacillus
✍
Stahly, Donald P. ;Ito, Junetsu
📂
Article
📅
1981
🏛
Springer
🌐
English
⚖ 274 KB