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Polyethylene-glycol induced fusion of bacterial protoplasts

✍ Scribed by Fodor, Katalin ;Alf�ldi, Lajos

Publisher
Springer
Year
1979
Tongue
English
Weight
451 KB
Volume
168
Category
Article
ISSN
0026-8925

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✦ Synopsis

Direct selection for recombinants by supplemented minimal media from polyethylene-glycol (PEG)-induced fusion of protoplasts of polyauxotrophic strains of B. megaterium revealed striking physiological influences on the yield of recombinants. Cytoplasmic state of the protoplasts to be fused, rather than genetic events, determined the number of colonies obtained on the selection media. It is suggested that the physiological effects primarily influenced the ability of the fused protoplasts to revert to bacillary form.

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