ADP-Ribosylation of Heterogeneous Ribonucleoproteins in HeLa Cells
β Scribed by S. Prasad; J. Walent; A. Dritschilo
- Book ID
- 115575552
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 400 KB
- Volume
- 204
- Category
- Article
- ISSN
- 0006-291X
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## Abstract ADPβribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with ^32^PβNAD; then the nucleolar proteins were analyzed by 1βdimensional and 2βdimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins
Ribonuclease activity in HeLa cell nuclei is markedly inhibited by ADP-ribosylation following incubation of intact isolated nuclei with [14C]NAD. Time course experiments demonstrate that [14C] incorporation into proteins is accompanied by a 50% inhibition of ribonuclease activity on single-strand an