ADP-Ribosylation in Permeable HeLa S3 Cells
✍ Scribed by Jostein HOLTLUND; Tom KRISTENSEN; Anne Carine ØTVOLD; Søren G. LALAND
- Book ID
- 115121576
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 805 KB
- Volume
- 130
- Category
- Article
- ISSN
- 1432-1327
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## Abstract ADP‐ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with ^32^P‐NAD; then the nucleolar proteins were analyzed by 1‐dimensional and 2‐dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins
Ribonuclease activity in HeLa cell nuclei is markedly inhibited by ADP-ribosylation following incubation of intact isolated nuclei with [14C]NAD. Time course experiments demonstrate that [14C] incorporation into proteins is accompanied by a 50% inhibition of ribonuclease activity on single-strand an