## Abstract ADPβribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with ^32^PβNAD; then the nucleolar proteins were analyzed by 1βdimensional and 2βdimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins
ADP-Ribosylation of Nonhistone Proteins in HeLa Cells: Modification of DNA Topoisomerase II
β Scribed by A.Ivana Scovassl; Cristina Mariani; Matteo Negroni; Claudia Negri; Umberto Bertazzoni
- Book ID
- 115601472
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 326 KB
- Volume
- 206
- Category
- Article
- ISSN
- 0014-4827
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Ribonuclease activity in HeLa cell nuclei is markedly inhibited by ADP-ribosylation following incubation of intact isolated nuclei with [14C]NAD. Time course experiments demonstrate that [14C] incorporation into proteins is accompanied by a 50% inhibition of ribonuclease activity on single-strand an
## Abstract The sensitivity of HeLa DNA topoisomerase II to 45Β°C heat shock was measured both in the intact cell and in vitro. In the intact cell, DNA topoisomerase II activity was estimated by measuring the formation and reversal of enzymeβDNA cleavable complexes by alkaline filter elution of cell