𝔖 Bobbio Scriptorium
✦   LIBER   ✦

ADP-ribosylation of nucleolar proteins in HeLa tumor cells

✍ Scribed by Norbert Leitinger; Jozefa Wesierska-Gadek

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
454 KB
Volume
52
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

ADP‐ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with ^32^P‐NAD; then the nucleolar proteins were analyzed by 1‐dimensional and 2‐dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE‐cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP‐ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP‐ribosylation.

📜 SIMILAR VOLUMES

Ethanol enhances ADP-ribosylation of pro
Ethanol enhances ADP-ribosylation of protein in rat hepatocytes
✍ B. Emmanuel Akinshola; Savitri Sharma; James J. Potter; Esteban Mezey 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 811 KB

Decreases in hepatocyte NAD+ produced by ethanol are only partially explained by the increased conversion of NAD+ to NADH and NADP+. The purpose of this study was to determine whether a mechanism for the ethanol-induced decrease in NAD+ is its increased use in ADP-ribosylation. Exposure of hepatocyt

How to kill tumor cells with inhibitors
How to kill tumor cells with inhibitors of poly(ADP-ribosyl)ation
✍ Aswin Mangerich; Alexander Bürkle 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 French ⚖ 455 KB 👁 1 views

## Abstract Poly(ADP‐ribosyl)ation is a post‐translational modification catalyzed by the enzyme family of poly(ADP‐ribose) polymerases (PARPs). PARPs exhibit pleiotropic cellular functions ranging from maintenance of genomic stability and chromatin remodeling to regulation of cell death, thereby re

A comparative study on the effect of tum
A comparative study on the effect of tumor promoters on poly ADP-ribosylation in A431 cells
✍ Neeta Singh 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 615 KB

## Abstract Poly ADP‐ribosylation is a post‐translational modification of chromatin proteins catalyzed by the enzyme poly‐ADPR transferase (poly‐ADPRT) and affects the structure as well as the functional properties of chromatin. It is of particular relevance in carcinogenesis, as it represents an e

Degradation of abnormal proteins in heLa
Degradation of abnormal proteins in heLa cells
✍ Klavs B. Hendil 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 524 KB

## Abstract The experiments show that abnormal proteins are degraded faster than normal ones in HeLa cells. Among the fragmentary proteins made in the presence of puromycin, those with low molecular weight are least stable. Proteins made after incubation with 5‐fluorouracil or in the presence of so

Degradation of abnormal proteins in HeLa
Degradation of abnormal proteins in HeLa cells
✍ Walter F. Prouty 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 845 KB

## Abstract Canavanine, an arginine analog, is incorporated into HeLa cell protein when cells are incubated in the absence of arginine, and this incorporation can result in the production of nonfunctional enzymes or abnormal proteins. The cells degrade these abnormal proteins up to three times more