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Degradation of abnormal proteins in HeLa cells

✍ Scribed by Walter F. Prouty

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
845 KB
Volume
88
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Canavanine, an arginine analog, is incorporated into HeLa cell protein when cells are incubated in the absence of arginine, and this incorporation can result in the production of nonfunctional enzymes or abnormal proteins. The cells degrade these abnormal proteins up to three times more rapidly than normal cell proteins. The capacity for selective degradation of abnormal proteins is not limited to HeLa cells since human fibroblasts also showed increased degradative rates following exposure to canavanine. In addition, enhanced degradation is not a peculiar property of canavanine incorporation since other amino acid analogs also promoted protein degradation. Thus, mammalian cells have the capacity to recognize and selectively degrade abnormal proteins.

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