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Activation of Nitric Oxide Synthase from Cultured Aortic Endothelial Cells by Phospholipids

✍ Scribed by Y. Ohashi; M. Katayama; K. Hirata; M. Suematsu; S. Kawashima; M. Yokoyama

Book ID: 115573562
Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 396 KB
Volume: 195
Category: Article
ISSN: 0006-291X
DOI: 10.1006/bbrc.1993.2187

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med