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Rapid activation of endothelial nitric oxide synthase by estrogen

✍ Scribed by Philip W. Shaul

Book ID: 117214064
Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 151 KB
Volume: 64
Category: Article
ISSN: 0039-128X
DOI: 10.1016/s0039-128x(98)00105-6

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med