Accurate measurements of long-range heteronuclear coupling constants from homonuclear 2D NMR spectra of isotope-enriched proteins
β Scribed by Gaetano T Montelione; Marjorie E Winkler; Peter Rauenbuehler; Gerhard Wagner
- Publisher
- Elsevier Science
- Year
- 1989
- Weight
- 477 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0022-2364
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π SIMILAR VOLUMES
Long-range heteronuclear coupling constants, n J XH , have been recognized as an important source of information in the structural elucidation of molecules (1). Accordingly, a large number of methods for their measurement have been proposed to date . Often these methods rely on the characteristic sp
A simple pulse sequence in inverse-detection mode is proposed for the detection of heteronuclear long-range couplings. In the resulting 2D heteronuclear multiple-bond correlation (HMBC) spectra, pure-absorptive peak patterns are obtained along both frequency dimensions. Since the heteronuclear coupl