โ Scribed by Koki Sato; Hiroko Hama-Inaba
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The temperature-sensitive (ts) Chinese hamster ovary (CHO) cell mutant tsHl contains a therrnolabile leucyl-tRNA synthetase. Upon incubation at the nonpermissive temperature of 39.5"C, the enzyme became reversibly inhibited over a period of minutes, and the cells lost viability over a period of many
## Abstract Mammalian cell populations may be enriched for temperatureโsensitive (__ts__) mutants by the tritiated thymidine (^3^HโTdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the nearโdiploid Chinese hamster ovary (CHO) cell line and on a line made โtetraploid
## Abstract A temperatureโsensitive growth mutant derived from the BHK 21 cell Line, ts AF8, was found to have greatly reduced DNA synthesis at the nonpermissive temperature. This reduction is mainly due to a decrease in the frequency of cells synthesizing DNA. Upon shift up, ts AF8 becomes blocked