A mammalian cell mutant with temperature-sensitive thymidine kinase
β Scribed by Michiko M. Nakano; Toyozo Sekiguchi; Masa -atsu Yamada
- Book ID
- 112558864
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 490 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1572-9931
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The temperature-sensitive (ts) Chinese hamster ovary (CHO) cell mutant tsHl contains a therrnolabile leucyl-tRNA synthetase. Upon incubation at the nonpermissive temperature of 39.5"C, the enzyme became reversibly inhibited over a period of minutes, and the cells lost viability over a period of many
## Abstract Mammalian cell populations may be enriched for temperatureβsensitive (__ts__) mutants by the tritiated thymidine (^3^HβTdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the nearβdiploid Chinese hamster ovary (CHO) cell line and on a line made βtetraploid