A nonradioactive multiwell spectrophotometric assay for the interferon-induced enzyme 2',5'-oligoadenylate synthetase measuring the inorganic pyrophosphate produced during oligoadenylate synthesis has been developed. A coupled enzymatic reaction results in a mole to mole formation of NADPH compared
โฆ LIBER โฆ
A Spectrophotometric Assay of Phosphoribosyl Pyrophosphate Synthetase
โ Scribed by Braven, J.; Hardwell, T. R.; Seddon, R.; Whittaker, M.
- Book ID
- 121468710
- Publisher
- Royal Society of Medicine Press
- Year
- 1984
- Tongue
- English
- Weight
- 593 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0004-5632
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A simple, rapid, and sensitive spectrophotometric assay of phosphoribosylpyrophosphate synthetase activity is described. This assay is based on the quantitative measurement of the reaction product AMP by a NADH-coupled enzyme method.