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A simplified procedure for the isolation of pig kidney d-amino acid oxidase

โœ Scribed by W.Terry Jenkins; Ann Behlen; Phillip J. Rogers

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
327 KB
Volume
94
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple, quick procedure for the isolation of pig kidney o-amino acid oxidase [EC 1.4.3.3; o-amino acid:oxygen oxidoreductase (deaminating)] is described based upon the use of granulated hydroxylapatite for chromatography. The purity appears to be comparable to that obtained by other procedures. The holoenzyme was isolated as the benzoate complex. ' A. B. was a participant in our NSF supported

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โœ Vincent Massey; Loren D. Mendelsohn ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 330 KB

The preparation of flavin adenine dinucleotide-aftinity columns employing glucose oxidase and D-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-bind