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Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution

✍ Scribed by Mikio Bakke; Chiaki Setoyama; Retsu Miura; Naoki Kajiyama

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
119 KB
Volume
93
Category
Article
ISSN
0006-3592

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## Abstract A new phytase (APPA) with optimum pH 2.5β€”substantially lower than that of most of microbial phytases (pH 4.5–6.0)β€”was cloned from __Yersinia frederiksenii__ and heterologously expressed in __Escherichia coli__. Containing the highly conserved motifs typical of histidine acid phosphatase