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A simplified method for the preparation of α-32P-labeled deoxyribonucleoside triphosphates

✍ Scribed by F. Wu; Y. Wang; M. Yi

Book ID
105441912
Publisher
Springer
Year
1997
Tongue
English
Weight
203 KB
Volume
218
Category
Article
ISSN
1588-2780

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A simple method for preparing s-32P-labeled nucleoside-5'-triphosphates of high specific activity is presented. Unprotected nucleosides are heated dry with urea and inorganic phosphate. The products from such reactions are phosphorylated enzymatically.