𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple procedure for the synthesis of α-32P-nucleoside triphosphates

✍ Scribed by Christof K. Biebricher


Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
306 KB
Volume
95
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis


A simple method for preparing s-32P-labeled nucleoside-5'-triphosphates of high specific activity is presented. Unprotected nucleosides are heated dry with urea and inorganic phosphate. The products from such reactions are phosphorylated enzymatically.


📜 SIMILAR VOLUMES


A simple method for synthesizing [γ-32P]
✍ Pál I. Bauer; Gyula Várady 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 256 KB

A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a

A simplified enzymic procedure for the p
✍ F. Maley; G.F. Maley; J.F. McGarrahan 📂 Article 📅 1967 🏛 Elsevier Science 🌐 English ⚖ 401 KB

The importance of nucleoside 5'-di-and triphosphates for the synthetic processes of the cell has led to the development of numerous chemical (l-3) and enzymic (47) methods for their preparation. Although these methods have obvious advantages, each is subject to limitations. Thus, the chemical proce

A Simple Procedure for the Synthesis of
✍ Žagar, Cyrill ;Scharf, Hans-Dieter 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 354 KB

## Abstract 3,4‐Di‐__O__‐benzyl‐2,6‐dideoxy‐L‐__arabino__‐hexono‐1,5‐lactone (1b) was synthesized in three steps by starting from di‐__O__‐acetyl‐L‐rhamnal (6). Sequential treatment of 1b or 3,4,6‐tri‐__O__‐benzyl‐2‐deoxy‐D‐__arabino__‐hexono‐1,5‐lactone (1a) with triethyloxonium tetrafluoroborate