A simple procedure for the synthesis of α-32P-nucleoside triphosphates
✍ Scribed by Christof K. Biebricher
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 306 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple method for preparing s-32P-labeled nucleoside-5'-triphosphates of high specific activity is presented. Unprotected nucleosides are heated dry with urea and inorganic phosphate. The products from such reactions are phosphorylated enzymatically.
📜 SIMILAR VOLUMES
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a
The importance of nucleoside 5'-di-and triphosphates for the synthetic processes of the cell has led to the development of numerous chemical (l-3) and enzymic (47) methods for their preparation. Although these methods have obvious advantages, each is subject to limitations. Thus, the chemical proce
## Abstract 3,4‐Di‐__O__‐benzyl‐2,6‐dideoxy‐L‐__arabino__‐hexono‐1,5‐lactone (1b) was synthesized in three steps by starting from di‐__O__‐acetyl‐L‐rhamnal (6). Sequential treatment of 1b or 3,4,6‐tri‐__O__‐benzyl‐2‐deoxy‐D‐__arabino__‐hexono‐1,5‐lactone (1a) with triethyloxonium tetrafluoroborate