## Centrifugation in density gradients is an important procedure for the fractionation and characterization of macromolecules and cellular organelles. A number of gradient-forming devices have been described (l-11), each with some desirable features. I now find that any one of several commercially
A simple monitoring system for density gradients
β Scribed by Roger J. Romani; L. Karl Fisher
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 687 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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One method of fractionating a density gradient after centrifugation is to puncture the bottom of the cent~fuge tube and then collect fractions of equal number of drops of gradient material. This method has its disadvantages. Occasionally air bubbles are introduced into the gradient
A miniaturized continuous gradient elution system with variable gradient profile was designed. A retention time reproducibility below 1% was achieved. The system was applied to the separation of phenolic compounds with a wide range of polarities.
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