✦ LIBER ✦

A simple system for rapid generation of duplicate density gradients

✍ Scribed by Donald F.H. Wallach

Publisher: Elsevier Science
Year: 1970
Tongue: English
Weight: 182 KB
Volume: 37
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(70)90268-x

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✦ Synopsis

Centrifugation

in density gradients is an important procedure for the fractionation and characterization of macromolecules and cellular organelles. A number of gradient-forming devices have been described (l-11), each with some desirable features. I now find that any one of several commercially available multichannel peristaltic pumps can be used to construct a simple and highly versatile gradient-forming system incorporating the following advantages: (1) rapid operation ; (.z?) capacity to form two (or more) identical gradients at a time; (3) great volume versatility, making it suitable for tubes and, potentially, for zonal rotors; (4) capacity to form linear, convex, and concave gradients. The basic principles of the system have been detailed by Wallach and Nordby (12) ; its application is shown here in the formation of small-volume gradients for membrane fractionation (13).

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