A simple microtechnique for isoelectric focusing on a density gradient
β Scribed by J.A. Kint
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 726 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A method for density gradient isoelectric focusing in small scale is described. It utilizes the same equipment as that ordinarily used for electrofocusing in polyacrylamide gels. thus allowing several samples to be analyzed in the same experiment and the simultaneous electrofocusing in density gradi
One method of fractionating a density gradient after centrifugation is to puncture the bottom of the cent~fuge tube and then collect fractions of equal number of drops of gradient material. This method has its disadvantages. Occasionally air bubbles are introduced into the gradient