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A method for density gradient isoelectric focusing in small scale

✍ Scribed by Jostein Holtlund; Tom Kristensen

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
465 KB
Volume
87
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for density gradient isoelectric focusing in small scale is described. It utilizes the same equipment as that ordinarily used for electrofocusing in polyacrylamide gels. thus allowing several samples to be analyzed in the same experiment and the simultaneous electrofocusing in density gradients and gels. Data concerning the stability of the pH gradient and the effect of salt and sample load on the focusing are given. Three different methods for fractionating the gradient are described; in one of these the frozen gradient is sliced with an ordinary gel slicer. This fractionation method is especiahy advantageous if the gradient contains zones of highly aggregated material, and it also allows the gradients to be stored frozen for later fractionation.

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