The substances to be separated must have an isoelectric point at which they are not charged.
Zonal electrophoresis and isoelectric focusing of proteins and virus particles in density gradients of small volume
β Scribed by B.D. Korant; K. Lonberg-Holm
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 445 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
On-line capillary zone electrophoresis/electrospray ionization mass spectrometry (CZE-ESMS) and capillary isoelectric focusing/electrospray ionization mass spectrometry (CIEF/ESMS) were employed for protein analysis. The separation mechanisms and the detection limits of CZE/ESMS and CIEF/ESMS were c
## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (MβIPG), onβline digestion by trypsinβbased immobilized enzyme microreactor (trypsinβIMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not