𝔖 Bobbio Scriptorium
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A new method for pH determination in isoelectric focusing experiments

✍ Scribed by C. Secchi

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
388 KB
Volume
51
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and reproducible method for the pH measurementin the effluent from density gradient electrofocusing is described. By this procedure, after preparative isoelectric focusing, the detection of protein zones and pH measurement can be accomplished simultaneously, by serially coupling a uv flow cell with a pH flow cell. This last one is connected to the recorder by a control unit, which allows the simultaneous printing of pH and uv absorption on the same chart.

Methods

Human hemoglobin, 98% pure, was prepared from freshly heparinized blood by the method of Adair and Adair ( 16). The hemoglobin solution was gassed with carbon monoxide to retard autooxidation and stored at 448

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