Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr
Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
β Scribed by Tiemin Huang; Janusz Pawliszyn
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 830 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (MβIPG), onβline digestion by trypsinβbased immobilized enzyme microreactor (trypsinβIMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not
The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha