๐”– Bobbio Scriptorium
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A simple density gradient generator

โœ Scribed by H.V. Samis Jr.


Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
278 KB
Volume
15
Category
Article
ISSN
0003-2697

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โœ Donald F.H. Wallach ๐Ÿ“‚ Article ๐Ÿ“… 1970 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 182 KB

## Centrifugation in density gradients is an important procedure for the fractionation and characterization of macromolecules and cellular organelles. A number of gradient-forming devices have been described (l-11), each with some desirable features. I now find that any one of several commercially

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โœ K.B. Tan ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 165 KB

One method of fractionating a density gradient after centrifugation is to puncture the bottom of the cent~fuge tube and then collect fractions of equal number of drops of gradient material. This method has its disadvantages. Occasionally air bubbles are introduced into the gradient

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Conventional large-volume gradient makers can be used in conjunction with peristaltic pumps to produce density gradients with total volumes as small as desired. The described approach involves continuous sampling of the large-volume gradient at a specific rate.