## Centrifugation in density gradients is an important procedure for the fractionation and characterization of macromolecules and cellular organelles. A number of gradient-forming devices have been described (l-11), each with some desirable features. I now find that any one of several commercially
A simple density gradient generator
โ Scribed by H.V. Samis Jr.
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 278 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
One method of fractionating a density gradient after centrifugation is to puncture the bottom of the cent~fuge tube and then collect fractions of equal number of drops of gradient material. This method has its disadvantages. Occasionally air bubbles are introduced into the gradient
## Birmingham Seotion. A SIMPLE EORJI Oh' GAS GENERA'I'OR. 1tY ,FIL\NK XIIEDDEN, U.SC., E1.C. 'We author cleacribcd n hrgc.si+ccl \Volrlcr iiplnratirs, for w e UH a pu gcncrntor for gcnenil laboratory purpoxes. It consists of n oylindricnl jiir 46-50 om. high anel 18 em. dinmeter, fitted wit11 R s
Conventional large-volume gradient makers can be used in conjunction with peristaltic pumps to produce density gradients with total volumes as small as desired. The described approach involves continuous sampling of the large-volume gradient at a specific rate.