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A simple micro-assay for inorganic phosphate

โœ Scribed by C.L. Penney

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
579 KB
Volume
75
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and samples containing less than 1 &ml of inorganic phosphate may be detected. This assay retains a unique principle of the former two, complexation (instead of reduction) of the phosphomolybdate heteropoly complex with an appropriate triphenylmethane dye (malachite green, methyl green). Use of detergents has been eliminated and some further properties of the dyes, the assay, and the latter's applicability to a coupled enzyme system for phosphomonoester and phosphodiester analysis are discussed. Consideration is also given to the associated phenomena of transphosphorylation. (

๐Ÿ“œ SIMILAR VOLUMES

A simple microassay for inorganic phosph
A simple microassay for inorganic phosphate, II
โœ C.L. Penney; Gordon Bolger ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 441 KB

Hydrochloric acid from Fisher assumed to be 12.0 N and appropriately diluted was determined by potentiometric titration to be 2.5 to 2.6 N.

An improved assay for nanomole amounts o
An improved assay for nanomole amounts of inorganic phosphate
โœ Peter A. Lanzetta; Lawrence J. Alvarez; Peter S. Reinach; Oscar A. Candia ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 247 KB

A calorimetric assay for the determination of nanomole amounts of inorganic phosphate is described. The procedure combines a very high molar extinction with color stability and insensitivity to newly released phosphate from labile organophosphates.