A simple enzymic assay for pyridoxal phosphate
โ Scribed by Bruce Chabner; David Livingston
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 503 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An enzymatic fluorometric assay to quantitate plasma pyridoxal 5'-phosphate (PLP) is described. PLP is preincubated for 30 min with purified tyrosine decarboxylase apoenzyme (TDA) in acetate buffer and is then incubated with L-tyrosine for 60 min. The decarboxylated metabolite, tyramine, is extracte
The alternate procedures used in the tyrosine apodecarboxylase assays for pyridoxal 5'-phosphate were evaluated to determine optimal conditions. Two preparations of tyrosine apodecarboxylase from Streptococcus faecalis were used: a cell suspension and a partially purified cell-free form. The activit
has allowed the development of a manual assay for inorganic phosphate of high simplicity and sensitivity. Total analysis requires only three reagents and is accomplished in less than 5 min, and samples containing less than 1 &ml of inorganic phosphate may be detected. This assay retains a unique pri
An assay for determining the concentration of pyridoxal Y-phosphate in plasma from 0.4 ml whole blood is reported. The assay consists of incubating deproteinized plasma with o-setine apodehydratase from Escherichia cob' in 0.5 M N-2-hydroxyethylpipetazine-N'-3-propesulfonic acid, pH 7.8, at 37ยฐC for