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A Robotics-Based Automated Assay for Inorganic and Organic Phosphates

✍ Scribed by Elizabeth B. Cogan; G.Bruce Birrell; O.Hayes Griffith

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
93 KB
Volume
271
Category
Article
ISSN
0003-2697

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Inorganic phosphate determination in the
Inorganic phosphate determination in the presence of a labile organic phosphate: Assay for carbamyl phosphate phosphatase activity
✍ Michael J. Black; Mary Ellen Jones πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 455 KB

An assay was developed for carbamyl phosphate phosphatase (CAP phosphatase) activity. This enzyme activity is difficult to assay because of the chemical instability of carbamyl phosphate (CAP). We chose to measure CAP phosphatase activity by monitoring release of inorganic phosphate (Pi). This entai