A new and convenient method for the determination of P, was developed. Phosphomolybdate is measured calorimetrically, without reduction to molybdenum blue, by dissolving the whole assay mixture in acetone, where phosphomolybdate is bright yellow. The hydrolysis of acidlabile phosphates (e.g., creati
A new enzymatic method for the determination of inorganic phosphate and its application to the nucleoside diphosphatase assay
β Scribed by Woo Ik Hwang; Sungman Cha
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 455 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The determination of enzyme activities and the screening of enzyme regulators is a major task in clinical chemistry and drug development. A broad variety of enzymatic reactions is associated with the consumption of adenosine triphosphate (ATP), including, in particular, phosphorylation
We present here an improvement in the classical molybdate method for inorganic phosphate determination. This method has high sensitivity, with 1 nmol Of Pi giving an absorbance change of 0.060 &a unit. It is highly reproducible and the color remains stable for at least 3.5 h. In addition the use of