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A simple assay for hyaluronidase activity using fluorescence polarization

โœ Scribed by Toshiyuki Murai; Hiroto Kawashima

Book ID
116297451
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
458 KB
Volume
376
Category
Article
ISSN
0006-291X

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A sensitive method for the assay of hyal
A sensitive method for the assay of hyaluronidase activity
โœ Leslie C. Benchetrit; Sham L. Pahuja; Ernest D. Gray; Ronald D. Edstrom ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 417 KB

A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (l-ethyl-2-[3-( l-ethyl-naphtho[l,M] to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is propo~ional to the amount