Hyaluronidase assay using fluorogenic hy
โ
Toshiya Nakamura; Mitsuo Majima; Kohmei Kubo; Keiichi Takagaki; Shinri Tamura; M
๐
Article
๐
1990
๐
Elsevier Science
๐
English
โ 450 KB
The reducing terminal of hyaluronate was labeled with a fluorogenic reagent, 2-aminopyridine. The pyridylaminohyaluronate was incubated with testicular hyaluronidase for 1 h. After incubation, 4 vol of ethanol was added to the incubation mixture, followed by centrifugation. The fluorescence of the s