A sensitive method for the assay of hyal
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Leslie C. Benchetrit; Sham L. Pahuja; Ernest D. Gray; Ronald D. Edstrom
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Article
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1977
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Elsevier Science
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English
โ 417 KB
A spectrophotometric method for the assay of hyaluronidase activity, based on the binding of a carbocyanine dye (l-ethyl-2-[3-( l-ethyl-naphtho[l,M] to undegraded substrate, is described. The binding results in a spectral shift with an absorbance maximum at 640 nm which is propo~ional to the amount